Journal: The journal of physical chemistry. C, Nanomaterials and interfaces

Article Title: Dye Encapsulation in Fluorescent Core-Shell Silica Nanoparticles as Probed by Fluorescence Correlation Spectroscopy

doi: 10.1021/acs.jpcc.9b00297

Figure Lengend Snippet: (a) Cy5 photo-induced cis-trans isomerization around the polymethinic chain, and corresponding kinetic model. (b) Schematic representation of step-wise synthesis of dye-encapsulating fluorescent core–shell SNPs. (c, d) GPC elugrams of as synthesized (c) and GPC purified (d) dye-encapsulating core–shell SNPs.

Article Snippet: Fluorescence correlation spectroscopy FCS setup A 635 nm (Cy5) continuous wave solid state laser (LDCU5/8283, Power Technology, Inc.) was reflected by a dichroic mirror and focused onto the object plane of a water immersion microscope objective (Zeiss Plan-Neofluar 63x NA 1.2).

Techniques: Synthesized, Purification

Journal: The journal of physical chemistry. C, Nanomaterials and interfaces

Article Title: Dye Encapsulation in Fluorescent Core-Shell Silica Nanoparticles as Probed by Fluorescence Correlation Spectroscopy

doi: 10.1021/acs.jpcc.9b00297

Figure Lengend Snippet: (a) Comparison of FCS results (with afterpulsing effects removed) for pure Cy5 (grey) and Cy5-encapsulating core-shell SNPs (colored) together with associated fits. (b) Particle diameter as a function of silica shell thickness as determined by translational diffusion (black) versus rotational diffusion (grey). (c) Dyes per particle as determined by a combination of FCS and absorption spectroscopy. (d) Brightness per particle as a function of silica shell thickness. (e) Brightness per encapsulated dye as a function of silica shell thickness. (f) Brightness enhancement per dye as a function of silica shell thickness. (g) Percentage of photoisomerizing dyes as determined by FCS as a function of silica shell thickness.

Article Snippet: Fluorescence correlation spectroscopy FCS setup A 635 nm (Cy5) continuous wave solid state laser (LDCU5/8283, Power Technology, Inc.) was reflected by a dichroic mirror and focused onto the object plane of a water immersion microscope objective (Zeiss Plan-Neofluar 63x NA 1.2).

Techniques: Comparison, Diffusion-based Assay, Spectroscopy

Journal: The journal of physical chemistry. C, Nanomaterials and interfaces

Article Title: Dye Encapsulation in Fluorescent Core-Shell Silica Nanoparticles as Probed by Fluorescence Correlation Spectroscopy

doi: 10.1021/acs.jpcc.9b00297

Figure Lengend Snippet: Fraction of Cy5 molecules being in the non-fluorescent cis conformation, P, relaxation time of Cy5 cis-trans isomerization, τ P , effective rate of isomerization from trans to cis, k iso , effective rate of back-isomerization form cis to trans, k biso , equilibrium constant of back-isomerization, K . Normalized values are with respect to Cy5 in water at 20 °C.

Article Snippet: Fluorescence correlation spectroscopy FCS setup A 635 nm (Cy5) continuous wave solid state laser (LDCU5/8283, Power Technology, Inc.) was reflected by a dichroic mirror and focused onto the object plane of a water immersion microscope objective (Zeiss Plan-Neofluar 63x NA 1.2).

Techniques:

Journal: The journal of physical chemistry. C, Nanomaterials and interfaces

Article Title: Dye Encapsulation in Fluorescent Core-Shell Silica Nanoparticles as Probed by Fluorescence Correlation Spectroscopy

doi: 10.1021/acs.jpcc.9b00297

Figure Lengend Snippet: (a) Comparison of fluorescence lifetimes of Cy5-encapsulating core-shell SNPs versus free Cy5 dye. (b) Intensity-matched absorption spectra of free Cy5 dye and Cy5-encapsulating fluorescent core−shell SNPs together with corresponding emission spectra. Emission spectra are normalized with respect to Cy5 in water.

Article Snippet: Fluorescence correlation spectroscopy FCS setup A 635 nm (Cy5) continuous wave solid state laser (LDCU5/8283, Power Technology, Inc.) was reflected by a dichroic mirror and focused onto the object plane of a water immersion microscope objective (Zeiss Plan-Neofluar 63x NA 1.2).

Techniques: Comparison, Fluorescence

Journal: The journal of physical chemistry. C, Nanomaterials and interfaces

Article Title: Dye Encapsulation in Fluorescent Core-Shell Silica Nanoparticles as Probed by Fluorescence Correlation Spectroscopy

doi: 10.1021/acs.jpcc.9b00297

Figure Lengend Snippet: Quantum yield as determined by FCS, φ F , fluorescence lifetime, τ F , radiative rate, k r , and non-radiative rate, k nr . Normalized values are with respect to Cy5 in water.

Article Snippet: Fluorescence correlation spectroscopy FCS setup A 635 nm (Cy5) continuous wave solid state laser (LDCU5/8283, Power Technology, Inc.) was reflected by a dichroic mirror and focused onto the object plane of a water immersion microscope objective (Zeiss Plan-Neofluar 63x NA 1.2).

Techniques: Fluorescence

Journal: The journal of physical chemistry. C, Nanomaterials and interfaces

Article Title: Dye Encapsulation in Fluorescent Core-Shell Silica Nanoparticles as Probed by Fluorescence Correlation Spectroscopy

doi: 10.1021/acs.jpcc.9b00297

Figure Lengend Snippet: Quantum yield as determined by steady-state absorption and emission spectroscopy, Φ F , fluorescence lifetime, τ F , radiative rate, k r , and non-radiative rate, k nr . For easy comparison, the quantum yield as obtained from FCS, φ F , and the fraction of Cy5 molecules being in the non-fluorescent cis conformation, P, are relisted. Normalized values are with respect to Cy5 in water.

Article Snippet: Fluorescence correlation spectroscopy FCS setup A 635 nm (Cy5) continuous wave solid state laser (LDCU5/8283, Power Technology, Inc.) was reflected by a dichroic mirror and focused onto the object plane of a water immersion microscope objective (Zeiss Plan-Neofluar 63x NA 1.2).

Techniques: Spectroscopy, Fluorescence, Comparison